Terrific Broth Medium
Steven J Burgess, is Sparrow
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Abstract
Tartoff, K.D. and Hobbs, C.A. (1987) Improved Media for Growing Plasmid and Cosmid Clones. Bethesda Research Laboratories Focus, 9, 12.
Steps
Prepare stock solutions
Combine the following components for 1L TB buffer
24g
20g
4mL Glycerol
Make up volume to 900mL with dH2O
Autoclave 121°C 0h 30m 0s
Prepare 1L of 10x TB Salts (0.17 M KH2PO4, 0.72 M K2HPO4) by combining the following
23.1g KH2PO4
125.4g K2HPO4
Make up volume to 1L with dH2O
Autoclave 121°C 0h 30m 0s
Prepare medium
Allow solutions to cool to 60°C then add
100mL of 10x TB salts to 900mL TB buffer.
Store at Room temperature
Practical use
We have found that using TB for plasmid preps consistently yields higher than using LB regardless of prep kit used, with half the volume of culture required.
It is not recommended to exceed ~2 mL of saturated TB culture per prep column due to overloading the column and not yielding clean preps.
