Direct delivery and fast-treatedAgrobacteriumco-culture (Fast-TrACC) plant transformation methods forNicotiana benthamiana

Jon P. Cody, Michael F. Maher, Ryan A. Nasti, Colby G. Starker, James C. Chamness, Daniel F. Voytas

Published: 2022-10-16 DOI: 10.1038/s41596-022-00749-9

genome editing

Agrobacterium

Fast-TrACC

plant transformation

Nicotiana benthamiana

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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There is an expanding need to modify plant genomes to create new plant germplasm that advances both basic and applied plant research. Most current methods for plant genome modification involve regenerating plants from genetically modified cells in tissue
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

Ishida, Y., Hiei, Y. & Komari, T. Agrobacterium-mediated transformation of maize. Nature 2, 1614–1621 (2007).
Altpeter, F. et al. Advancing crop transformation in the era of genome editing. Plant Cell 28, 1510–1520 (2016).
Lowe, K. et al. Rapid genotype ‘independent’ Zea mays L. (maize) transformation via direct somatic embryogenesis. Vitr. Cell. Dev. Biol. Plant 54, 240–252 (2018).
Nelson-Vasilchik, K., Hague, J., Mookkan, M., Zhang, Z. J. & Kausch, A. Transformation of recalcitrant sorghum varieties facilitated by Baby Boom and Wuschel2. Curr. Protoc. Plant Biol. 3, e20076 (2018).
Gordon-Kamm, B. et al. Using morphogenic genes to improve recovery and regeneration of transgenic plants. Plants Basel Switz. 8, 38 (2019).
Lowe, K. et al. Morphogenic regulators Baby boom and Wuschel improve monocot transformation. Plant Cell 28, 1998–2015 (2016).
Maher, M. F. et al. Plant gene editing through de novo induction of meristems. Nat. Biotechnol. 38, 84–89 (2020).
Xie, K., Minkenberg, B. & Yang, Y. Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc. Natl Acad. Sci. USA 112, 3570–3575 (2015).
Čermák, T. et al. A multipurpose toolkit to enable advanced genome engineering in plants. Plant Cell 29, 1196–1217 (2017).
Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A modular cloning system for standardized assembly of multigene constructs. PLoS ONE 6, e16765 (2011).
Baltes, N. J., Gil-Humanes, J., Cermak, T., Atkins, P. A. & Voytas, D. F. DNA replicons for plant genome engineering. Plant Cell 26, 151–163 (2014).
Anzalone, A. V. et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature 576, 149–157 (2019).
Gordon-Kamm, B. et al. Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants. Berleigh Dodds Ser. Agric. Sci. 1–36 (2021).
Parrot, W. A. et al. Recovery of primary transformants of soybean. Plant Cell Rep. 7, 615–617 (1989).
Yan, B. et al. Agrobacterium tumefaciens-mediated transformation of soybean [Glycine max (L.) Merril.] using immature zygotic cotyledon explants. Plant Cell Rep. 19, 1090–1097 (2000).
Matsuda, N. et al. Development of an Agrobaterium-mediated transformation method for pear (Pyrus communis L.) with leaf-section and axillary shoot-meristem explants. Plant Cell Rep. 24, 45–51 (2005).
Engler, D. E. & Grogan, R. G. Isolation, culture and regeneration of lettuce leaf mesophyll protoplasts. Plant Sci. Lett. 28, 223–229 (1983).
Li, J. et al. Multiplexed, targeted gene editing in Nicotiana benthamiana for glyco-engineering and monoclonalantibody production. Plant Biotechnol. J. 14, 533–542 (2016).
Li, Z. et al. High frequency generation of fertile transgenic rice plants after PEG-mediated protoplast transformation. Plant Mol. Biol. Rep. 8, 276–291 (1990).
Rao, K. S. et al. In planta transformation of pigeon pea: a method to overcome recalcitrancy of the crop to regeneration in vitro. Physiol. Mol. Biol. Plants 14, 321–328 (2008).
Ganguly, S. et al. Development of transgenic pigeonpea using high throughput plumular meristem transformation method. Plant Cell Tissue Org. Cult. 135, 73–83 (2018).
Ganguly, S. et al. Plumular meristem transformation system for chickpea: an efficient method to overcome recalcitrant tissue culture responses. Plant Cell Tissue Org. Cult. 142, 493–504 (2020).
Sparkes, I. A., Runions, J., Kearns, A. & Hawes, C. Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants. Nat. Protoc. 1, 2019–2025 (2006).
Barton, M. K. Twenty years on: the inner workings of the shoot apical meristem, a developmental dynamo. Dev. Biol. 341, 95–113 (2010).
Hoerster, G. et al. Use of non-integrating Zm-Wus2 vectors to enhance maize transformation: non-integrating WUS2 enhances transformation. Vitr. Cell. Dev. Biol. Plant 56, 265–279 (2020).
Doyle, J. J. & Doyle, J. L. A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19, 11–15 (1987).
Brinkman, E. K., Chen, T., Amendola, M. & van Steensel, B. Easy quantitative assessment of genome editing by sequence trace decomposition. Nucleic Acids Res. 42, e168 (2014).

Direct delivery and fast-treatedAgrobacteriumco-culture (Fast-TrACC) plant transformation methods forNicotiana benthamiana

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