A versatile tissue-rolling technique for spatial-omics analyses of the entire murine gastrointestinal tract

Gustavo Monasterio, Rodrigo A. Morales, David A. Bejarano, Xesús M. Abalo, Jennifer Fransson, Ludvig Larsson, Andreas Schlitzer, Joakim Lundeberg, Srustidhar Das, Eduardo J. Villablanca

Published: 2024-06-20 DOI: 10.1038/s41596-024-01001-2

spatial-omics

GI tract

tissue-rolling

multiplexed staining

transcriptomic profiling

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

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A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
Acknowledgements

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A versatile tissue-rolling technique for spatial-omics analyses of the entire murine gastrointestinal tract

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