The present work analyzes the antineoplastic effects of molecular iodine alone and in combination with the neoadjuvant therapy FEC/TE (5-fluorouracil/epirubicin/cyclophosphamide or taxotere/epirubicin) in women with breast cancer. In this protocol the immunohistochemistry methodology for the quantification of the estrogen receptor and the proteins associated with the installation of the mesenchymal epithelium transition (e-cadherin and vimentin), immune response (CD8) and cell death (TUNEL) is described, and the methodology for transcriptomic analyses includes: RNA-Seq and transcriptomic analysis; pathway enrichment analysis; validation of the immune response and epithelium/stroma rate and, prediction of the transcription factor regulatory network and peroxisome proliferator-activated receptors type gamma (PPARg) interaction

Molecular iodine protocols.docx