Transgenic mouse colony maintenance
Ariadna Laguna, Miquel Vila, Iria Carballo-Carbajal
Published: 2024-06-18 DOI: 10.17504/protocols.io.yxmvmeyx5g3p/v1
AI 解读 浏览 1313
Abstract
Methods for the generation and maintenance of transgenic tyrosinase mice
Steps
1.
Pronuclear microinjection of the human
tyrosinase complementary DNA fused to the rat tyrosine hydroxylase promoter into C57Bl6-SJL (RRID:MGI:5656718) mouse zygotes
2.
Selection of positive lines and backcrossing for 8-10 generations using
C57BL/6J mice (Charles River; RRID:MGI:3028467)
3.
Maintainance in heterozygosis by crossing heterozygous males with C57BL76J females
4.
Housed two to five per cage with ad libitum
access to food and water during a 12 hours (h) light/dark cycle (light-on 8
a.m.)
推荐阅读
Nature Protocols
Protocols IO
Current Protocols
