Loading Syringes to Inject into Microfluidics Device
freeman.lan
Abstract
Protocol for loading syringes with small amounts of liquid sample for injection into droplet microfluidic devices.
Steps
Preparing Syringes
Take out a syringe
Equipment
| Value | Label |
|---|---|
| Luer Lok Syringe (1mL) | NAME |
| BD Biosciences | BRAND |
| 309628 | SKU |
| http://www.bd.com/en-us | LINK |
Add 27g needle to the syringe
Equipment
| Value | Label |
|---|---|
| Precisionglide Needle | NAME |
| BD | BRAND |
| 301629 | SKU |
| http://www.bd.com/en-us | LINK |
Use the needle and syringe to draw out approximately 200µL of
Cut approximately 20 centimeters of
Loading the Syringe
Push the syringe so that the HFE7500 oil starts dripping at end of the tubing.
Put the tube into the solution that you would like to load, and slowly draw the syringe to pull the solution into the tubing and syringe.
Interfacing with the Device
Attach the loaded syringes to the syringe pumps
Equipment
| Value | Label |
|---|---|
| NE-300 Just Infusion™ Syringe Pump | NAME |
| Automatic syringe pump | TYPE |
| New Era Pump Systems, Inc. | BRAND |
| NE-300 | SKU |
Insert the other end of the tubing into the appropriate hole in the microfluidic device, using a tweezer or by hand.
Set the appropriate syringe diameter and pump rates into the syringe pump and press start. You may want to begin the pumps with a higher pump rate in order to get the syringes to pressurize more quickly.
