10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation -- University of Minnesota TMCs (CG000478 Rev B)
IOx Genomics, Laura J Niedernhofer, David A Bernlohr
Abstract
DOIs for dissociation protocols and 10x Genomics fixation for Chromium Single Cell Expression flex protocols.
Please see DOIs for dissociation protocols linked here.
Protocol ID# (CG) and Revision letter provided here :
10x CG000478, **Revision B** -- Fixation of single cells and nuclei prior to applying 10x Genomics Chromium flex protocols.
Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
Steps
http://www.10xgenomics.com/products/single-cell-gene-expression-flex
http://www.10xgenomics.com/support/single-cell-gene-expression-flex
10x Protocol CG000478, Rev B (Fixation):
10x Cell+Nuclei Fixation Chromium Fixed RNA Profiling, Rev B (Protocol CG000478).pdf
